Fernando H. do Nascimento, et al. Talanta, 2020, 217, 121063.
Polymer monolithic columns were prepared inside fluorinated ethylene propylene (FEP) tubing by UV-initiated grafting to activate the inner surface wall. The obtained columns have permeability and reproducible chromatographic parameters and can be used for the separation of proteins by reversed-phase liquid chromatography.
Preparation procedure of FEP-based polymer monolithic columns
· To begin with, the FEP tubes (1.50 mm i.d.) are cleaned with ethanol and acetone, and then dried under N2. A solution of benzophenone (BP) in methanol is sonicated and purged with N2 before filling the tubes, which are then closed and irradiated under UV light for 60 minutes. The tubes are then washed with methanol, dried, and filled with a solution of ethylene glycol dimethacrylate (EDMA) in methanol, which is sonicated and purged with N2. After filling the tubes, they are closed and irradiated again under UV light for 20 minutes.
· Once the inner walls of the FEP tubes are functionalized, a polymerization mixture is prepared in the presence of DMAP. This mixture is sonicated and bubbled with N2 before filling the activated FEP tubes. The tubes are closed and irradiated under UV light for 20 minutes. After polymerization, the column ends are cut to provide the desired length, and the columns are flushed with ACN at a constant pressure.