What is the recommended pore size for proteins larger than 20kDa in Western blotting?
The recommended pore size for proteins larger than 20kDa in Western blotting is 0.45μm.
What is the recommended pore size for imprinted proteins less than 20kDa in Western blotting?
The recommended pore size for imprinted proteins less than 20kDa in Western blotting is 0.2μm.
What are PVDF transfer membranes resistant to?
PVDF transfer membranes are resistant to most organic solvents, acids, and weak bases.
What are PVDF transfer membranes compatible with?
PVDF transfer membranes are compatible with commonly used transfer conditions and detection methods, such as staining, chemiluminescence, radiolabeling, etc.
How is the PVDF membrane prewetted?
The PVDF membrane is prewetted by methanol.
What are the advantages of PVDF transfer membranes compared to nitrocellulose membranes?
The advantages of PVDF transfer membranes compared to nitrocellulose membranes include good mechanical strength, strong protein retention, low background, extensive solvent compatibility, and excellent coloring ability.
What is the purpose of Western blotting?
The purpose of Western blotting is to analyze and quantify proteins, identify and semi-quantitatively analyze specific proteins.
How can the protein be separated in Western blotting?
The protein is separated by gel electrophoresis.
What are the steps involved in the Western blotting protocol?
The steps involved in the Western blotting protocol are cell culture, protein extraction, concentration and exchange, SDS-PAGE electrophoresis, membrane transfer, blocking and antibody incubation, and protein detection.
What is the sensitivity of PVDF transfer membranes in Western blotting?
PVDF transfer membranes have excellent sensitivity in Western blotting.
